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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ACLY All Species: 36.06
Human Site: S446 Identified Species: 72.12
UniProt: P53396 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P53396 NP_001087.2 1101 120839 S446 L N A S G S T S T P A P S R T
Chimpanzee Pan troglodytes XP_511495 1775 198342 S553 L N A S G S T S T P A P S R T
Rhesus Macaque Macaca mulatta XP_001108114 1137 124370 S500 L N A S G S T S T P A P S R T
Dog Lupus familis XP_850198 1101 120705 S446 L N A S G S T S T P A P S R T
Cat Felis silvestris
Mouse Mus musculus Q91V92 1091 119709 S446 L N A S G S T S T P A P S R T
Rat Rattus norvegicus P16638 1100 120617 S445 L N A S G S T S T P A P S R T
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus NP_001025711 1101 121113 S446 L N A S G S P S T P A P S R T
Frog Xenopus laevis NP_001088452 1101 120767 S446 L N A S G S S S T P A P S R T
Zebra Danio Brachydanio rerio NP_001002649 1092 119691 T446 L N A S G G T T T P A S S R S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_523755 1112 121387 Q448 L P G G Q Q A Q A D L K A A S
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P53585 1106 121598 G458 L S P E R N T G G T E R A P P
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q8X097 670 72608 T79 D F I C K R S T P S V A G I I
Conservation
Percent
Protein Identity: 100 59.9 90.3 98.1 N.A. 97.1 97.5 N.A. N.A. 93.1 89.3 86.3 N.A. 69.3 N.A. 60.8 N.A.
Protein Similarity: 100 61.1 92.3 99.4 N.A. 98.3 98.6 N.A. N.A. 96.5 95.3 93 N.A. 81.5 N.A. 76.5 N.A.
P-Site Identity: 100 100 100 100 N.A. 100 100 N.A. N.A. 93.3 93.3 73.3 N.A. 6.6 N.A. 13.3 N.A.
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. N.A. 93.3 100 86.6 N.A. 20 N.A. 33.3 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. 39.9
Protein Similarity: N.A. N.A. N.A. N.A. N.A. 49.7
P-Site Identity: N.A. N.A. N.A. N.A. N.A. 0
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. 13.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 75 0 0 0 9 0 9 0 75 9 17 9 0 % A
% Cys: 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 9 0 0 0 0 0 0 0 0 9 0 0 0 0 0 % D
% Glu: 0 0 0 9 0 0 0 0 0 0 9 0 0 0 0 % E
% Phe: 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 9 9 75 9 0 9 9 0 0 0 9 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 9 0 0 0 0 0 0 0 0 0 0 9 9 % I
% Lys: 0 0 0 0 9 0 0 0 0 0 0 9 0 0 0 % K
% Leu: 92 0 0 0 0 0 0 0 0 0 9 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 75 0 0 0 9 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 9 9 0 0 0 9 0 9 75 0 67 0 9 9 % P
% Gln: 0 0 0 0 9 9 0 9 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 9 9 0 0 0 0 0 9 0 75 0 % R
% Ser: 0 9 0 75 0 67 17 67 0 9 0 9 75 0 17 % S
% Thr: 0 0 0 0 0 0 67 17 75 9 0 0 0 0 67 % T
% Val: 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _